Extended Data Fig. 7: SRS images of larvae brain samples from flies fed on different diets.
a. DO-SRS images of a drosophila larvae brain in 3x glucose group. The wide range new lipid (CD) and old lipid (CH2) signal show the distribution of newly synthesized lipids and old lipids in whole sample, respectively. In the zoomed-in images, the microscopic distribution of two different lipid components is clearly shown. After deconvolution, the nanoscopic distribution and shape of lipid droplets are getting clearer. By using the particle analysis method, we can remove the background and focus on the areas of lipid droplets. b. SRS images of a drosophila larvae brain in the control group were processed with the same manner in a. These images were analyzed, and the analysis result is explained in Fig. 6.
