Extended Data Fig. 5: RNA-sequencing of fs-LM and dissociation-FACS isolated neurons. | Nature Methods

Extended Data Fig. 5: RNA-sequencing of fs-LM and dissociation-FACS isolated neurons.

From: Femtosecond laser microdissection for isolation of regenerating C. elegans neurons for single-cell RNA sequencing

Extended Data Fig. 5

a, b, Number of genes detected at different sequencing depth obtained by subsampling 19 million total reads from bulk (each bulk sample has >5,000 cells) and single neuron samples. c, Cumulative distribution of sequencing depth among all single-neuron samples collected with fs-LM and verified using scRNA-seq library quality checks. Single-neuron samples below the red dotted line were discarded due to insufficient sequencing depth. d, Number of genes identified in fs-LM isolated single neurons (n = 46 uninjured PLM neurons) and single TRNs isolated using dissociation-FACS method (n = 23 single TRNs from WT animals). e, f, Top tissue enrichment analysis terms of fs-LM isolated single neurons (e) and dissociation-FACS isolated single neurons (f). Data presented as mean + standard error of mean (SEM). g, Correlation of gene expression profiles between the ensembles of single TRNs (isolated using FACS-dissociation method, n = 23) and the single PLM neurons (isolated using the fs-LM method, n = 40).

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