Extended Data Table. 1 Average of mean FRET efficiency and standard deviation for MalE and U2AF2 samples reported by the participating laboratories

From: Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

  1. The calculated average \(\mu _{\left\langle E \right\rangle }\) and standard deviation \(\sigma _{\left\langle E \right\rangle }\) of the mean FRET efficiency values provided by the participating labs are given for all three studied mutants of MalE labeled with Alexa546 and Alexa647 under both apo and holo conditions (see Supplementary Table 3). The calculated mean and standard deviation of the difference in the reported mean FRET efficiency between the apo and holo (\(\left\langle {E_{{{{\mathrm{holo}}}}}}\right\rangle - \left\langle{E_{{{{\mathrm{apo}}}}}} \right\rangle\)) for the three MalE mutants are given by\(\mu _{\left\langle {E_{{{{\mathrm{holo}}}}}}\right\rangle - \left\langle{E_{{{{\mathrm{apo}}}}}} \right\rangle }\) and \({{{\mathrm{\sigma }}}}_{\left\langle {E_{{{{\mathrm{holo}}}}}}\right\rangle - \left\langle{E_{{{{\mathrm{apo}}}}}} \right\rangle }\) respectively (see Supplementary Table 3). The calculated average \(\mu _{R_{\left\langle E \right\rangle }}\) and standard deviation \(\sigma _{R_{\left\langle E \right\rangle }}\) of the mean distances were derived according to Eq. 2. The modeled distances \(R_{\left\langle E \right\rangle }^{{{{\mathrm{AV}}}}}\) and \(R_{\left\langle E \right\rangle }^{{{{\mathrm{ACV}}}}}\) are derived using accessible volume (AV) and accessible contact volume (ACV) calculations respectively, as described in the Methods. We also give the average and standard deviation for the FRET values determined for U2AF2 labeled with Atto532-Atto643 under both apo and holo conditions (Supplementary Table 4).
  2. *Only studied by two laboratories.
  3. **Due to the fast-structural dynamics in the sample, only 7 labs studied this mutant and distances were not determined.
  4. *** Only the holo state under holo condition was considered.
Back to article page