Extended Data Fig. 9: Evolution path for mScarlet3 (seq-id #15) and mScarlet-I3 (#16) from mScarlet (#1) and mScarlet-I (#2), respectively.
From: mScarlet3: a brilliant and fast-maturing red fluorescent protein
The numbers correspond with the sequence IDs in Supplementary Fig. 1. The horizontal axis depicts the (modulation) fluorescence lifetime as determined in live HeLa cells ~ 24 h after transfection and corresponds with intrinsic brightness (more to the right is better), and the vertical axis depicts the cellular brightness (higher is better) normalized to mScarlet-I. F. Vertical error bars are SD of the slope of the line fitted to the red to cyan cellular ratios (of 22–77 cells, for example see Extended Data Fig. 1) or the average modulation lifetime of the mScarlet variant in HeLa cells (from 3 individual FLIM recordings with > 5 cells each).
