Extended Data Fig. 6: Relative cell viability of RFP transfected cells.
From: mScarlet3: a brilliant and fast-maturing red fluorescent protein
mEGFP and RFPs were transfected separately into HeLa cells at day 0. At day 2 each of the RFP transfected cells were split and mixed with mEGFP transfected cells. At days 4 and 6 the cell mixtures were resplit. At days 2, 4 and 6 after (4–5 h after splitting/mixing) the number of RFP and mEGFP expressing cells in the mixtures was scored. The ratio of RFP to mEGFP expressing cells was normalized to that obtained at day 2 to correct for bias in transfection efficiencies. For each RFP 8-12 technical replicates (depicted as individual data points) were measured. The average normalized ratio of red to green cell numbers ± sd is indicated.
