Extended Data Table 1 Properties of red fluorescent proteins

From: mScarlet3: a brilliant and fast-maturing red fluorescent protein

  1. aAbsorbance maximum.
  2. bEmission maximum.
  3. cExtinction coefficient at maximum absorbance.
  4. dQuantum yield.
  5. eFluorescence lifetime component 1.
  6. fFluorescence lifetime component 2.
  7. gAmplitude of fluorescence lifetime component 1.
  8. hChi-square fluorescence lifetime fit.
  9. iAverage fluorescence lifetime weighed by amplitude.
  10. jModulation lifetime in cells.
  11. kApparent pKa value.
  12. lHill coefficient.
  13. mCalculated intrinsic brightness, product of extinction coefficient and quantum yield.
  14. nBrightness in mammalian cells normalized to mScarlet-I.
  15. oTime in seconds to half the emission rate under wide-field conditions.
  16. pTime in seconds to half the emission rate under confocal spinning disk conditions.
  17. qPercentage of photochromic behavior.
  18. rAccumulation in cells normalized to mScarlet-I (brightness in cells divided by intrinsic brightness).
  19. sApparent delay time of maturation relative to mTurquoise2 in mammalian cells.
  20. tInitial fluorescence at pH 13.5 relative to pH 7.4.
  21. uTime required to reduce fluorescence by 50% at pH 13.5. Sensitized emission by FRET in a RFP-SYFP2 fusion, corrected for direct excitation of donor and acceptor, normalized to mScarlet-I.
  22. wPercentage of cells with a correct ER labeling upon expressing an Cyterm-RFP fusion.
  23. xPercentage of cells visible OSER structures upon expressing an Cyterm-RFP fusion.
  24. yNumber of analyzed cells.
  25. zValues from Semiakina et al.. c,d,e,f,j,k,n,m,n,q,r,s ± standard deviation, or value cited from literature. Values in Italics from Bindels et al.9.
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