Fig. 1: OMAPs enhance standardization, discovery and stewardship of resources used in the spatial mapping of tissues at single-cell resolution.

a, Bar graph depicting the number of AS, CT and BP covered by each OMAP. Numbers were calculated by comparing with the relevant ASCT+B table⁶ and were reviewed by domain experts. b, Plot showing the cost to develop (filled circles) and use (unfilled circle) the OMAPs described here. Costs (in 2022 US dollars) are shown for antibodies and/or conjugation kits and exclude labor, amortization of purchased equipment such as microscopes, and consumables other than antibodies and conjugation kits. Cost to use is calculated for 100 tests. DNA (CODEX), Fluorophore (IBEX), Fluorophore* (Cell DIVE), Metal (SIMS). c, Schematic detailing the creation of a base OMAP panel. Gray antibodies indicate antibodies that do not pass the first quality control check for accurate immunolabeling. Colored antibodies outside of the filter reflect validated antibodies that are not suitable for the final OMAP, for example, need amplification or a different conjugate. d, Images of human lymph node depicting AS, CT and cell states identified using IBEX. GC, Tfr (follicular regulatory T cells), Treg (regulatory T cells), TBM (tingible body macrophages) and R1–R4 refer to different regions in the secondary follicle. 3⁺⁺ indicates CD69⁺⁺, ICOS⁺⁺ and PD-1⁺⁺; 1⁺ indicates CD69⁺ or ICOS+ or PD-1⁺. Large insets, 150 µm; small insets, 50 µm. Representative of a dataset of ten samples.