Fig. 3: Electric field stimulation-induced NEMO responses in rat hippocampal neurons.
From: Engineering of NEMO as calcium indicators with large dynamics and high sensitivity
a, Average Ca2+ responses reported by GECIs, 1 Hz stimulation (mean traces, n = 10–11 cells). b,c, Mean NEMOf and GCaMP6f transients induced by 5 Hz (b, left, mean traces; right, statistics; P = 0.86, unpaired Student’s t-test, two-tailed, n = 12 cells) or 10 Hz (c, mean traces, n = 11 cells) stimulation. Data are shown as mean ± s.e.m.; NS, nonsignificant. d, Mean GECI responses elicited by stimulation at varied frequencies. Inset, enlarged views of responses of reference GECI sensors (SBR magnified by nine times). e, Statistics for data shown in a and d. Each GECI measurement set was analyzed from several dendrites of at least ten neurons in three different primary hippocampal neuron cultures. All data in this figure are shown as mean ± s.e.m. (for stimulation at varied frequencies, GCaMP6s, n = 10, 10, 10, 11, 10 cells; GCaMP6f, n = 10, 11, 11, 11, 10, 10 cells; jGCaMP8f, n = 10, 10, 11, 11, 11 cells; NEMOm, n = 10, 12, 12, 13, 12 cells; NEMOs, n = 10, 10, 11, 14, 10 cells; NEMOf, n = 11, 11, 11, 12, 14 cells; NEMOc, n = 11, 11, 11, 13, 12 cells).
