Extended Data Fig. 10: NativeMS spectra of semisweet from a. detergent, b. bilayer with PE, PG without CL, c. bilayer with PE, PG, and 2.5% CL. | Nature Methods

Extended Data Fig. 10: NativeMS spectra of semisweet from a. detergent, b. bilayer with PE, PG without CL, c. bilayer with PE, PG, and 2.5% CL.

From: Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer

Extended Data Fig. 10

The individual peaks were plotted in the Origin software and then the area under the curve was obtained by fitting the peak with constant baseline mode. The 6+ monomer charge state and 9+ and 11+ dimer charge states were considered for oligomer % calculation. The peak areas of protein and lipid-bound protein from Origin software were then taken for percentage calculations and the final bar graph of this calculation is shown as an inset in a. The data clearly shows increase in CL% increases the dimeric population in the bilayer. The inset on the left side in each panel shows the mass plot obtained from UniDec analysis.

Source data

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