Extended Data Fig. 7: Spectra of VAMP2 showing no SM/EPC binding.

Spectra of VAMP2 from SV-like lipid vesicles where all the PC were replaced by a, SM and b, EPC (lipid compositions shown in the respective inset tables). The arrows showing the absence of theoretically predicted SM/EPC bound m/z values. In both the cases, under the same MS conditions, no detectable amount of SM or EPC binding was observed. All measurements were replicated over three independent measurements.