Extended Data Fig. 6: Similarity at TAD level for GAM, bulk Hi-C and sc-Hi-C.
From: Multiplex-GAM: genome-wide identification of chromatin contacts yields insights overlooked by Hi-C
a, Comparison of topological associated domains (TADs) defined by the insulation score method in GAM (top) and Hi-C6 (bottom). b, Overlap of TAD boundaries detected in GAM-1250 (red) and Hi-C (blue) as well as boundaries detected in sc-Hi-C contact maps produced from 50 cells, 100 cells, and all 588 cells7. c, Overlap between TAD boundary calls from 1NP GAM data with Hi-C data and full 1+3NP GAM-1250 data. d, Boundary strength (drop of insulation) measured for GAM (red), Hi-C (blue) and sc-Hi-C contact maps produced from 50 cells, 100 cells, and all 588 cells was calculated for TAD boundaries which are shared between the sets (dark bars) or only found in a single set (light bars). e, Mean frequency and 95% bootstrap CI (shaded) of RNA-Pol II, transcription factor occupancy and epigenetic marks centered at TAD boundary sites. Common, Hi-C-specific, GAM-specific boundaries are shown in black, dark blue, and red, respectively, gray values are based on shuffled boundary positions of each category.
