Extended Data Fig. 2: Ex-ST captures RNA with limited biases.
a, Comparison of the number of UMIs per gene between two Ex-ST MOB replicates. b-d, Comparisons of the number of UMIs per gene detected in the Ex-ST and standard Visium MOB datasets (b), in the Ex-ST and bulk RNA-seq MOB datasets (c) and in the Ex-ST and Slide-seq hippocampus datasets (d). Red lines: least squares linear fit. e, Pie charts showing RNA biotypes captured by Ex-ST and Visium using standard and modified protocols from MOB samples. Proportions are quantified by the total UMI counts of each biotype. f, Gene body coverage for the Ex-ST and standard/modified Visium MOB data. g, Bioanalyzer traces of cDNA from MOB samples before the fragmentation step for the Ex-ST and standard/modified Visium protocols. h, Ratio of exonic and intronic reads in the Ex-ST and standard/modified Visium MOB data.
