Extended Data Fig. 3: Annotation of spatial clusters of the MOB Ex-ST data. | Nature Methods

Extended Data Fig. 3: Annotation of spatial clusters of the MOB Ex-ST data.

From: Expansion spatial transcriptomics

Extended Data Fig. 3

a, The expression of region-specific marker genes detected by ISH, obtained from Allen Brain Atlas10. b, Comparison of number of UMIs of select region-specific marker genes measured by Ex-ST and standard Visium from matched tissue areas. Bar charts: mean of two replicates. c, UMAP showing Ex-ST MOB clusters identified by unsupervised clustering, colored by cluster annotation. d, Marker gene expression overlaid on the UMAP visualization. e, Dot plot showing top marker genes for each cluster.

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