Extended Data Fig. 6: Ex-ST hippocampal data has higher spatial resolution compared to standard Visium.

a, Cell type composition of individual spots in the Ex-ST data downsampled to match the sequencing saturation of the 10x Genomics dataset. Three major neuronal cell types are shown. Note that the patterns are almost identical to Fig. 2b, suggesting that cell type devolution is mostly insensitive to sequencing depth in the regime of 60–90% saturation. b, Comparison of ISH (top), obtained from Allen Brain Atlas¹⁰, normalized spatial expression of oligodendrocyte marker genes detected by Ex-ST (middle) and standard Visium (bottom). c, Cell type composition of individual spots in Ex-ST downsampled to match the sequencing saturation of the 10x Genomics data. Oligodendrocyte subclasses are shown, with patterns identical to Fig. 2e. d, Comparison of normalized spatial distribution of low-expression dendrite-enriched transcripts between Ex-ST and standard Visium. Note the lack of pattern in the standard Visium data.