Extended Data Table 1 Sample information obtained from space ranger software (10x Genomics) output

From: Expansion spatial transcriptomics

  1. Visium mouse olfactory bulb (MOB) datasets obtained using the standard and modified protocols were collected on tissue sections containing two bulbs. Both bulbs are combined to compare with the expanded MOB data with two replicates each containing one bulb. Downsampled Ex-ST and standard Visium MOB data have sequencing saturation matching with the data generated using the modified protocol. Hippocampus data include matched tissue areas from coronal sections of the mouse brain in the hippocampal region. Downsampled Ex-ST hippocampus data have sequencing saturation matching with the 10x Genomics hippocampus data. With matched sequencing saturation, the Ex-ST data always contain fewer reads per spot compared with the Visium datasets. This is expected given that each spot in the Ex-ST data corresponds to fewer cells and therefore should have lower library complexity.