Extended Data Fig. 8: Processing scheme for trEM GroEL-GroES data.

a, Masked 3D classification was performed after consensus 3D refinement of pooled data for time points 50, 200 ms, and 20 s. The particles were classified into 20 classes. Ten classes with a substantial number of particles are shown out of which classes displaying high-resolution features were retained. Each of these classes was split into subsets corresponding to individual timepoint. b-e, Reconstructions from data subsets corresponding to individual time points for panel a, classes 4, 9, 14, and 15, respectively. f, Processing scheme for 13 ms timepoint. A consensus volume map was 3D classified with a mask into 6 classes. g, Data from 13 and 50 ms timepoints were merged, a consensus map was auto-refined with C7 symmetry, and particles were classified into 8 classes. Three classes converged to high resolution and are shown in the scheme. Class 1 and class 8 were split into 13 and 50 ms subsets to generate time point-specific individual volumes.