Extended Data Fig. 2: Evaluation of in-drop mixing efficiency.
From: Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting
a, Experimental design: serpentine channel with 16 sections was used to evaluate mixing efficiency. Position 0 is the position at which two solutions flow parallel to each other before droplet formation. Pressures applied to the oil and water phase from top to bottom were 80, 85; 120,115; and 160, 140 mbar. Positions used for evaluating the relative mixing index (RMI) are indicated. The images show representative snapshots from high-speed videos containing > 50 frames each. b, Pixel intensity histograms of the imaged droplets qualitatively indicate the mixing extent. The histograms represent pixel intensity distributions within identical areas of the water-in-oil droplet at the indicated positions. c, RMI for three flow conditions calculated at eight selected positions. Data are presented as mean values ± s.d. Each point of the graph was obtained analyzing three droplets.
