Extended Data Fig. 5: Resident alveolar type II cells, with GFP-labeled surfactant protein C (green) can be imaged inside the crystal ribcage. | Nature Methods

Extended Data Fig. 5: Resident alveolar type II cells, with GFP-labeled surfactant protein C (green) can be imaged inside the crystal ribcage.

From: Crystal ribcage: a platform for probing real-time lung function at cellular resolution

Extended Data Fig. 5

(a) Stereomicroscope fitted with a NightSea GFP filter to visualize the SPC-GFP distribution at the whole organ scale. (b) Confocal microscopy of the SPC-GFP population at the lobe scale on the right side of the lung at 1.25x magnification. Vessels are labeled by injection of 50 µL of 10 mg/ml Evans blue dye in saline in vivo prior to extraction of the lungs. (c) Confocal microscopy at the 10x magnification shows single SPC-GFP+ cells in the lung distal parenchyma. (d) Single cell nuclei and SPC-GFP signal in cell cytoplasm visible at single cell resolution using a 60x water immersion lens.

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