Extended Data Fig. 7: Single cell deformation in response to changing alveolar pressure.
From: Crystal ribcage: a platform for probing real-time lung function at cellular resolution
(a) Cell-scale confocal microscopy of the lung inside the crystal ribcage with a FITC-albumin vascular lumen label showed the shadow of the cancer cell (b) which was imaged in a separate channel and merged to show cancer cells stretched in capillaries compared to arterioles and venules. (c) XY- views of the cancer cell at different depths of confocal imaging to show the cell is completely enclosed in the capillary. (d, e) Cells in capillaries quantified by the Feret ratio trended towards greater deformation in comparison to cells in larger arterioles-venules. Feret ratio quantified for n = 40 cells in capillaries, n = 30 cells in arteriole-venule at each alveolar pressure and data presented as mean ± SEM.
