Extended Data Fig. 4: Dynamic ventilation of mouse lung shows alveoli remain at a larger diameter for longer duration at higher ventilation rate.
From: Crystal ribcage: a platform for probing real-time lung function at cellular resolution
Comparing the same region of the lung ventilated at (a) 60 breaths/minute with (b) 120 breaths/minute by (c) the mean ± SEM alveolar diameter from m=4 ROI over n = 2 mice with a normalized ventilation cycle. Blue traces in the inset images show the segmentation boundary drawn to quantify single alveoli. (d) The width of the normalized ventilation cycle from m=4 ROI over n = 2 mice at each respiratory rate are statistically larger at the higher respiratory rate. Boxplots present median with 25th and 75th percentiles, whiskers are the maximum and minimum data points not considered outliers. Comparison between groups performed with two-tailed Student’s t-test for significance < 0.05.
