Fig. 4: Heterogeneity analysis of the Sc80S ribosome (EMPIAR: 10002).
From: OPUS-DSD: deep structural disentanglement for cryo-EM single-particle analysis
a, UMAP visualization of the eight-dimensional latent space of all particles encoded by OPUS-DSD. Solid black dots represent the cluster centers for labeled classes. b, Sixteen density maps reconstructed by the decoder of OPUS-DSD using the Kmeans clustering centers in its eight-dimensional latent space as inputs. The different locations of two RNA strands in different conformations are highlighted by the red solid and dashed boxes, respectively. c, Superimposition of class 1 and class 6 to show the displacement of 40S subunits highlighted in the red dashed boxes. Red arrows indicate the displacement directions. 60S is highlighted in a green dashed ellipse. d, The density map of class 9 is more complete than that reconstructed using all particles. Both maps are contoured at the same level and visualized by ChimeraX.
