Extended Data Fig. 1: Density maps of the pre-catalytic spliceosome reconstructed by OPUS-DSD’s decoder.
From: OPUS-DSD: deep structural disentanglement for cryo-EM single-particle analysis
a). Twenty density maps of the pre-catalytic spliceosome generated by OPUS-DSD’s decoder at cluster centers shown in Fig. 2a as inputs. b). Comparisons between densities of SF3b, Helicase and Core in Class 12 from OPUS-DSD and high-resolution structure from multi-body refinement, together with the consensus model. Class 12 is shown in green and transparent. The high-resolution structures from multi-body refinement are shown in solid colors inside Class 12. Left-to-right three columns: the comparisons focusing on SF3b (purple), Helicase (red) and Core/Foot (yellow), respectively. The consensus structure is shown in solid blue on the right as a reference.
