Extended Data Fig. 2: Identifying developmental progression with Twin Networks.
From: Uncovering developmental time and tempo using deep learning
Comparison of two subsequently acquired images of the same embryo. Similarity plots calculated by comparison with reference images differ minimally with respect to the peak of similarity as well as similarity to distant embryonic stages. Subtracting the similarity of the earlier acquired image (turquoise) from the similarity profile of the later acquired image (purple) shows positivity following the peak of the similarity (blue), suggesting the attribution of the later acquired image towards later developmental stages. Comparisons of subsequent images taken 3 min and 8 seconds apart are shown for images of embryos captured at 5.1 hpf in (a) and of subsequent images of embryos captured at 7.8 hpf in (b). Two images per plot from the acquisition of one embryo, representative for three independent experiments, are shown. The temporal limits for this approach have not yet been defined. Embryos for illustration are also shown in Fig. 1c,e. Scale bars, 500 μm.
