Fig. 2: System-wide quantification of RNA localization.
From: System-wide analysis of RNA and protein subcellular localization dynamics
a, Schematic representation of how localization proportions are estimated. New profile (left) is decomposed into different proportions of each marker profile (middle) to approximate the original profile (right). b, Cytosol (Cyt), membrane (Mem) and nuclear (including nucleolus) (Nuc) proportions for all transcripts (left) and well-documented localization markers (right). c, Schematic representation of cell fractionation by sedimentation coefficient and linear profiles of the localization markers. d, Principal component analysis projections of RNA profiles, with markers highlighted. e, Membrane, nucleus and cytosol proportions obtained by equilibrium density centrifugation-based LoRNA projected on the sedimentation-based RNA localization results. f, Correlations between proportions obtained using different fractionation approaches. Cytosol proportion by density is the sum of cytosol and cytosol-light proportions. Membrane proportion by sedimentation is the sum of mitochondrial and ER proportions.
