Extended Data Fig. 1: mEnrich-seq of urine samples using Nanopore Flongle flow cells.
From: mEnrich-seq: methylation-guided enrichment sequencing of bacterial taxa of interest from microbiome
a, Three urine samples were sequenced as the input: UTI-1 contained mostly bacterial DNA, while UTI-2 and UTI-3 were dominated by human DNA. b, Scatter plots for six urine samples (UTI-1 to -6) showing the percentage of reads mapped to E. coli, human and other taxa from the input (x-axis) vs. mEnrich-seq (y-axis). The UTI-1 to -3 samples demonstrate consistent patterns as shown in Fig. 2 in the main text (MinION flow cell); the additional three UTI urine samples (UTI -4 to -6) are included to illustrate the consistent enrichment of E. coli by mEnrich-seq. Across all six samples, E. coli reads are significantly enriched in mEnrich-seq. In UTI-2, UTI-3, UTI-4, and UTI-6, the proportion of reads corresponding to the human genome are significantly lower due to cleavage by DpnII in mEnrich-seq. c, Fold enrichment of E. coli reads from mEnrich-seq compared to the input for the six urine samples.
