Extended Data Fig. 2: mEnrich-seq data of UTI-1 include reads that have high-confidence mapping to E. coli yet carry additional genes beyond the isolated strain from standard urine culture.
From: mEnrich-seq: methylation-guided enrichment sequencing of bacterial taxa of interest from microbiome
a, Top: 10 reads with highly confident mapping to E. coli from mEnrich-seq of UTI-1 urine sample, consistently supporting a tet(A) resistant gene (from AF534183, blue boxes). For each read, lengths of the sequences flanking the resistance gene were shown on both sides. The identities between the reads and tet(A) were shown inside the blue boxes. Bottom, reads alignment to tet(A) was performed with Clustalw2 and colored with MView. b, Validation of tet(A) in UTI-1 sample. Agarose gel electrophoresis of the tet(A) regions amplified once by two sets of primers (of lengths 1150 bp and 900 bp, respectively). The bands (in red square) were further purified for gene sequence confirmation by Sanger sequencing (Supplementary Information).
