Fig. 2: μkiss labeling and lipid diffusion in a model membrane. | Nature Methods

Fig. 2: μkiss labeling and lipid diffusion in a model membrane.

From: A paintbrush for delivery of nanoparticles and molecules to live cells with precise spatiotemporal control

Fig. 2

a, Schematic showing a synthetic membrane of DOPC–DOPS with a low concentration of GM1 approached by the brush micropipette pair. The close-up shows an impression of clustered GM1 and its labeling by CTxB-AF488. Inset shows SEM of the ⌀in = 1 μm micropipette pair, with the μkiss envelope shown by the dashed line. Scale bar, 1 μm. b, Steps of a diffusion experiment. First, the flow is switched on, depositing CTxB-AF488 on the membrane (i). After 1 s of operation, flow is switched off (ii) and CTxB-AF488-labeled lipids diffuse (iii). c, Confocal fluorescence image series of the sequence in (b(i–iii)), representative of a recorded series of size n = 30. Scale bars 1 μm. d, Peak fluorescence amplitude of a μkiss labeling and FRAP measurement as a function of time. a.u., arbitrary units. e, Measured diffusion constants for μkiss and FRAP (green) with their mean and standard deviation (black), each from a series of size n = 30.

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