Fig. 5: Visualizing intercellular forces at a junction. | Nature Methods

Fig. 5: Visualizing intercellular forces at a junction.

From: A paintbrush for delivery of nanoparticles and molecules to live cells with precise spatiotemporal control

Fig. 5

a, Fluorescence image of a linear ensemble of cells with mEmerald-labeled actin filaments. Targeted cell and application zone are marked. b, Image of two cells following Lat-A treatment for 25 min to the application zone shown in a. Orange arrowheads act as reference markers throughout the figure for the cell–cell boundary. Black arrowheads denote a region of gap formation, discussed later. Observation is indicative of results obtained from a series of two measurements. Scale bars 10 μm. c, Close-up view of the cell–cell contact boundary 4 min after first exposure to Lat-A. Black arrows mark the site of gap formation. Scale bar 5 μm. d, Kymograph showing an increase in the gap size over time along the line marked in c.

Back to article page