Extended Data Fig. 8: Comparison of gGRAB5-HT3.0 and other green 5-HT sensors during reward and tone delivery.
From: Improved green and red GRAB sensors for monitoring spatiotemporal serotonin release in vivo
a, Schematic illustrates the experimental design. b–c, Representative pseudocolor images (left) and averaged traces (right) of fluorescence signals (z-score) from g5-HT3.0 and iSeroSnFR in a mouse exposed to 5% glucose (b) or 2-s tone (c) conditions. The dashed line indicates the delivery of water or tone. d, Group analysis of the area under the curve (AUC) of fluorescence signals from g5-HT3.0 and iSeroSnFR in response to the application of 5% glucose or 2-s tone conditions. Two-tailed paired t-tests, P = 2.4 × 10−5 and 0.46 for glucose and tone, respectively. e–g, Representative pseudocolor images (left), averaged traces (right) and AUC group data (g) of fluorescence signals from g5-HT3.0 and g5-HT1.0 during exposure to 5% glucose (e) or 2-s (f) tone conditions, similar to panels b–d. Two-tailed paired t-tests in g, P = 4.4 × 10−4 and 0.632 for glucose and tone, respectively. n = 40 trials from 4 mice for each group. Data are shown as mean ± s.e.m. in b–g, with the error bars or shaded regions indicating the s.e.m. ***P < 0.001, n.s., not significant.
