Extended Data Fig. 4: PDRG1 coelution profile in the PFDN2 and UXT DIP-MS experiments.
From: DIP-MS: ultra-deep interaction proteomics for the deconvolution of protein complexes
PDRG1 shows three distinct peaks (labelled 1,2,3) in the DIP-MS experiments of PFDN2, whereas in the UXT DIP-MS only two peaks (1,2) are identified. In both DIP-MS experiments the two peaks at the higher MW belong to the PFDL complex (2) and the PFDL containing PAQosome complex (1). In the PFDN2 DIP-MS the third (3) and most intense PDRG1 peak coelutes with canonical PFD. The intensity shows the smoothed average MS2 protein intensity normalized to the maximum protein intensity across all gel-slices. PFDN4 and PFDN5 coeluting at the PFD peak (dashed lines in the UXT DIP-MS) are contaminant signal in the UXT DIP-MS experiment. The signal is of PFDN4 and PFDN5 is three orders of magnitude lower compared to the PFDN2 signal at the PFDL complex.
