Extended Data Fig. 3: Kilosort false positives from the Spikeinterface paper are due to unrealistically long spike durations.

a, Segment of a real Neuropixels recording used for comparison. High-pass filtering was applied at 100Hz (rather than 300 Hz) to better illustrate true spike durations. b, Same as a for a segment of a biophysical simulation. cd, Waveforms extracted from the real and simulated recordings respectively. Waveforms were superimposed across channels, using a color code to illustrate channels further from the center (yellow means further). To measure spike duration and spatial extent we used trough-to-peak (T2P) and max-distance at half-max (MDHM) respectively. e, Distribution of waveform statistics from the real recording and simulation (n = 292 for real data and n= 250 for simulations, center lines indicate median and error bars indicate 5 and 95 percentiles). f, False positive clusters reported by spikeinterface are mostly spikes that were temporally split due to their very long durations. g, Benchmarks for original biophysical simulation and a 2x sped up version. h, Number of false positives decreases when the simulation is sped up 2x, which makes spike durations more similar to real recordings.