Extended Data Fig. 1: Principle and schematic of TICO microscope.
From: Large-scale deep tissue voltage imaging with targeted-illumination confocal microscopy
(a-c) Design principle of TICO microscope: (a) conventional strategy for incorporation a DMD into a confocal microscope; (b) bypassing DMD in the detection path to avoid fluorescence loss; (c) inserting a wedge prism in front of the DMD corrects for both image plane tilt and 1D magnification change caused by the DMD, restoring confocality between excitation and detection beams. (d) Detailed schematic of TICO microscope. DM, dichromatic mirror. Em, emission filter. Ex, excitation filter. PBS, polarizing beam splitter. λ/2, half-wave plate. λ/4, quarter-wave plate. Galvo, galvanometric scanner. DMD, digital micromirror device. Obj, objective.
