Fig. 3: Comparison of diffusion of data generated by different platforms.

a, Expression markers include Slc17a7 in the mouse olfactory bulb (left), Ptgds in the mouse brain (middle) and Pmel in the E12.5 eye (right). The plots are based on raw count values. Black boxes indicate the selected regions used for diffusion calculation. b, Expression levels of the aforementioned marker genes (from a) are aggregated for every 10 μm along 50 μm in the olfactory bulb, 500 μm in the brain and 300 μm in the eyes, as shown in a. UMI counts are averaged across modalities, normalized for each platform and presented in a density plot with the area under the curve set to 1 (details in Methods). c, Expression level of the marker genes as mentioned above (from a) within selected modalities are provided, with black dashed lines delineating the boundaries used for diffusion calculations. d, The left-width at half-maximum (LWHM) of the profile was then calculated for each gene (from a) in each modality and displayed in boxplots. Each dot represents the LWHM for a given modality (n = 6 for olfactory bulb and brain, n = 3 for eye). Modalities for which LWHM could not be calculated were removed. The box denotes the interquartile range, the range between the 25th and 75th percentile, with the median value; whiskers indicate the maximum and minimum value within 1.5 times the interquartile range.