Fig. 2: Reconstruction of complete mouse neurons using CAR.
From: Collaborative augmented reconstruction of 3D neuron morphology in mouse and human brains
a, Complete reconstruction of example mouse neurons from 20 different brain regions. Top left, top–down view of example neurons registered to the standard Allen Brain Atlas. Each color represents an individual neuron, and the inset on the right indicates the respective brain region to which these neurons belong. Bottom and right, visualization of the neurons separately, providing their type, reconstruction accuracy, number of bifurcations (#Bif) and total length (len; μm). The mapped morphology in the standard atlas and the brain region that the neuron originates in are also visualized below each neuron. AM, anteromedial nucleus; AUDv, ventral auditory area; CM, central medial nucleus of the thalamus; CP, caudoputamen; LD, lateral dorsal nucleus; LGd, lateral geniculate complex, dorsal part; LP, lateral posterior nucleus; MG, medial geniculate complex; MOp, primary motor area; MOs, secondary motor area; PVT, paraventricular nucleus; RSPv, ventral part; RT, reticular nucleus; SI, substantia innominata; SSp.m, primary somatosensory area, mouth; SSs, supplemental somatosensory area; VISp, primary visual area; VPM, ventral posteromedial nucleus. b, Projection map illustrates the lengths of reconstructed neurites contributed through collaborations. The horizontal and vertical axes represent the origin (soma location) and destination (projection location) regions, respectively. Each cell in the map represents a projection pair, with the darkness of shading corresponding to the amount of the cross-edited length by collaboration. A ‘+’ symbol (yellow) is employed to denote cases in which collaborative addition was the predominant operation, while a ‘−’ symbol (purple) is used for instances in which collaborative subtraction dominated the editing process. The symbol ‘o’ indicates that no editing was performed through collaboration. The detailed names of the brain regions can be found in Methods. c, NTH of recon nodes of matched and unmatched structures for 20 neurons. This plot compares the topological height of reconstructed nodes with expert results at eight stages along the reconstruction timeline. Matched structures (bottom) indicate successful reconstructions that align with expert results, while unmatched structures (top) deviate from expert results. d, Average accuracy and user consistency for 20 neurons across eight tracing stages. Blue polyline, average accuracy (blue shading, 95% confidence interval); orange polyline, agreement among the contributors (orange shading, 95% confidence interval).
