Fig. 5: Reconstruction of subneuronal structures using CAR.
From: Collaborative augmented reconstruction of 3D neuron morphology in mouse and human brains
a, Left, on the right side, a top–down view (CCFv3) of 156,190 semi-automatically annotated somas is presented, depicting six selected brain regions color coded along the anterior–posterior axis (left). Corresponding soma locations are plotted as dots (right). The different colors of the somas represent different users who contributed to the annotations. Right, close-up views of the eight selected regions mentioned in a, left. Auto-traced dendrites are also shown for those neurons for which somas have been annotated by different users. The six selected regions include the main olfactory bulb (MOB), anterior olfactory nucleus (AON), nucleus accumbens (ACB), the CP, field CA1 (CA1) and the inferior colliculus, external nucleus (ICe). The panel displays the number of annotated somas and users involved in the region. b, Top left, sagittal view of 20 neurons with boutons that were generated and proofread by CAR. Each distinct color represents boutons from different brain regions. Bottom, three image blocks (maximum intensity projection in 2D is shown), denoted as R1, R2 and R3, which were selected for evaluation (scale bar, 10 μm). Potential presynaptic sites that were auto-detected and validated are marked with green markers, while the white markers indicate incorrectly detected boutons that were deleted by users. Missing boutons spotted by the four users are shown in pink, azure, blue and yellow, respectively. Top right, the precision, recall and F1 scores for these three selected image regions.
