Extended Data Fig. 5: Detection and structure determination of RuBisCo enzymes from cellular tomograms.
From: MiLoPYP: self-supervised molecular pattern mining and particle localization in situ
a) 2D grid visualization of embeddings obtained from tilt series downloaded from EMPIAR-10694 (top, left). RuBisCo particles are seen as the most prominent feature (yellow/green). Membrane-like features (magenta) are also observed. Tomographic slice colored according to the embedding coordinates (top, right). A zoomed-in view highlighted in white is also provided. A selection of patches containing membrane (magenta) and RuBisCo particles (lime) are shown below. b) Annotated tomogram with detected RuBisCo particles colored in red (left). Zoomed-in view showing the accurate localization of RuBisCo particles (top, right). 11.0 Å resolution reconstruction of RuBisCo obtained from 35,352 particles with PDB coordinates 1GK8 fit into the map (bottom, right).
