Extended Data Fig. 7: Post-processing pipeline for tubular-shaped protein detection.
From: MiLoPYP: self-supervised molecular pattern mining and particle localization in situ
a) Tomographic slice showing tubular structures observed in tilt series from EMPIAR-10987 obtained from in situ lamellae (left). Output heatmap from the particle localization module with bright areas representing detected microtubules (middle). Output coordinates (green circles) for detected particles prior to post-processing (right). False positive regions are highlighted in red. b) The post-processing stage is composed of three steps: 1) convert 3D coordinates into a graph representation and identify connected components within the graph (left), 2) polynomial curve fitting for each connected component and evaluation of the quality of fit (middle), and 3) curves with fitting values above a user-defined threshold and with curvature smaller than a preset threshold are extracted as the final coordinates representing microtubules (right).
