Fig. 3: Structural mapping and accurate identification of native ribosomes inside bacterial cells.
From: MiLoPYP: self-supervised molecular pattern mining and particle localization in situ
a, 2D grid visualization of embeddings obtained from tilt series downloaded from EMPIAR-10499 (top, left) showing ribosomes (blue), cellular membranes (purple/red) and other smaller-sized proteins (dark green). 3D tomogram visualization view (top, right) with voxels colored according to the embedding coordinates. A selection of patches containing 70S ribosomes is shown below (purple outline). b, A partially annotated input tomogram corresponding to 70S ribosomes (blue circles) used for training the refinement module (left). The trained model accurately identifies ribosomes (red circles) while ignoring membrane and background areas (right). c, 5.4 Å resolution SPT reconstruction of the 70S ribosome obtained from a total of 17,381 particles detected from 65 tomograms.
