Fig. 5: Simultaneous identification and structure determination of multiple protein species from in situ lamellae.
From: MiLoPYP: self-supervised molecular pattern mining and particle localization in situ
a, 2D grid visualization of in situ tomograms from EMPIAR-10987 (top, left). The embedding space shows a variety of features and proteins including 80S ribosomes (purple) and microtubules (green). 3D tomogram visualization with voxels colored according to the embedding coordinates in the 2D grid visualization (top, right). A selection of patches containing microtubules (bottom, left) and 80S ribosomes (bottom, right) are shown. b, After training and inference of the refinement module, MiLoPYP accurately localizes 80S ribosomes (purple) and microtubules (green). c, 8.6 Å resolution reconstruction of 80S ribosomes obtained from 4,570 particles. d, 37.0 Å resolution reconstruction of microtubules from 1,761 particles. PDB coordinates 6MTB and 6RZB were fitted into the maps using rigid body fitting, respectively.
