Fig. 6: Detection and structure determination of ATP synthase from in situ lamellae.
From: MiLoPYP: self-supervised molecular pattern mining and particle localization in situ
a, 2D grid visualization of tomograms from EMPIAR-11658 (left). The embedding space shows a variety of features and proteins including ATP synthase (green). Over-clustering with labels shows a group of patches corresponding to ATP synthase complexes (top, right). A selection of patches containing ATP synthase (bottom, left). b, Selected tomogram reconstruction with segmentation and the ATP synthase structure mapped back into the original positions. c, 13.0 Å resolution structure of ATP synthase obtained from 2,577 particles with PDB coordinates 8FL8 fitted into the map using rigid body fitting.
