Extended Data Fig. 6: 3D SIM reconstruction of a live F-Actin labeled RPE-1 cell. | Nature Methods

Extended Data Fig. 6: 3D SIM reconstruction of a live F-Actin labeled RPE-1 cell.

From: Neural space–time model for dynamic multi-shot imaging

Extended Data Fig. 6

a, Maximum z-projection of the reconstructed volume with color-coded depth. b, Zoom-in comparisons, from left to right: conventional reconstruction2, NSTM reconstruction, NSTM reconstruction with color-coded time (three colors for three illumination orientations), and widefield images with color-coded time. The second row of each zoom-in assumes raw images with longer delay between orientations, Δt(ori.), and thus more motion (that is, the raw images of orientation 1 are from acquisition timepoint 1, orientation 2 from acquisition timepoint 2, and orientation 3 from timepoint 3 from a time-series measurement).

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