Fig. 3: 3D SIM reconstruction of a live RPE-1 cell expressing StayGold-tagged mitochondrial matrix protein.
From: Neural space–time model for dynamic multi-shot imaging
a, Maximum projection of the volume with color-coded depth. b,c, Zoom-in of a slice from the 3D reconstruction, comparing the conventional 3D SIM algorithm (CUDA-accelerated three-beam SIM reconstruction software2) with our NSTM algorithm. The NSTM reconstruction disambiguates the artifacts induced by tubular motion (as indicated by the arrows). d,e, The NSTM reconstructions and widefield images at three timepoints coded by colors. Widefield images are obtained by summing the raw images from five phase shifts.
