Extended Data Fig. 5: 3D Axis-on-a-chip for identifying cell-cell interactions.
From: Differentiating visceral sensory ganglion organoids from induced pluripotent stem cells
(a) Entire immunostaining image of axis-on-a-chip indicating VSGO chamber by TUJ1 (red) and HCO chamber by DSG2 (yellow) (Representative image among 3 biological replicates). (b and c) Diffusion test was conducted in microfluidic chip using 10 µM of 40 kDa FITC-dextran solution. Fluorescence images showing diffusion of dextran within the microchannel when with no ECM hydrogel or with ECM hydrogel composed of Collagen I and matrigel. (d) Graph represents relative intensity comparison of dextran fluorescence of each condition. (e) PRV-infected colon organoid connected with neurite bundle of VSGO. PRV expressing GFP and neurite immunostained with TUJ1 (red) (Representative image among 3 biological replicates). ECM, Extracellular matrix; PRV, pseudorabies virus. Scale bars indicate 500 µm (a), 50 µm (b), 200 µm (c and d).
