Extended Data Fig. 3: Functional characterization of VSGOs.
From: Differentiating visceral sensory ganglion organoids from induced pluripotent stem cells
(a) Representative live-cell images before and after treatment with capsaicin (10 µM) and liraglutide (10 µM) (Baseline indicates before treatment) (Representative images among 2 batches, Scale bars: 100μm). (b) Schematic of timeline for calcium imaging. (c) Venn diagram representing number of i-VSNs activated by capsaicin or liraglutide or both. (d) Schematic of timeline for patch-clamp recording. (e) Bright field image showing patch-clamp experiment on VSN (Representative image among 3 batches, Scale bars: 10μm). (f) The examples of voltage traces. (g) Increasing relationship of spike number vs current (total 26 cells analyzed among 3 batches, mean ± SEM). (h and i) i-VSN was activated responding to capsaicin (h) and GLP1R agonist DMB (i). (j) Venn diagram of i-VSN number ratio responding to capsaicin or DMB, or both.
