Voltage imaging, a promising technique for directly recording neuronal activity, faces barriers to broad application due to current limitations in compatible imaging modalities. Our team introduces an advanced confocal light field microscopy method enabling high-throughput, rapid and low-noise 3D voltage imaging in awake mice.
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References
Knöpfel, T. & Song, C. Optical voltage imaging in neurons: moving from technology development to practical tool. Nat. Rev. Neurosci. 20, 719–727 (2019). This review article provides an overview of genetic encoded voltage indicators (GEVIs) and voltage imaging techniques.
Quicke, P. et al. Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators. Neurophotonics 7, 035006 (2020). This paper demonstrates subcellular-resolution GEVI light field imaging in acute mouse brain slices, resolving dendritic voltage signals localized in 3D.
Zhang, Z. et al. Imaging volumetric dynamics at high speed in mouse and zebrafish brain with confocal light field microscopy. Nat. Biotechnol. 39, 74–83 (2021). This paper reports the development of confocal LFM for calcium imaging and blood flow imaging.
Pnevmatikakis, E. A. et al. Simultaneous denoising, deconvolution, and demixing of calcium imaging data. Neuron 89, 285–299 (2016). This paper describes an algorithm with a constrained deconvolution approach for neural activity extraction.
Fan, L. Z. et al. All-optical electrophysiology reveals the role of lateral inhibition in sensory processing in cortical layer 1. Cell 180, 521–535 (2020). This paper describes a targeted illumination wide-field microscope for voltage imaging that uses a spatial light modulator.
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This is a summary of: Bai, L. et al. Volumetric voltage imaging of neuronal populations in the mouse brain by confocal light-field microscopy. Nat. Methods https://doi.org/10.1038/s41592-024-02458-5 (2024).
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Mapping voltage activity in a live mouse brain in 3D using confocal light field microscopy. Nat Methods 21, 1992–1993 (2024). https://doi.org/10.1038/s41592-024-02459-4
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DOI: https://doi.org/10.1038/s41592-024-02459-4