Fig. 4: Reglycosylation of CD16b.

a, The structure of the CD16b (PDB 1E4J, resolution 2.50 Å) with N-glycosylation sequons labeled. The bold labels indicate occupied sequons in neutrophil-bound CD16b⁵³. The green check marks indicate the sequons predicted to be occupied by the Re-Glyco GlcNAc-Scanning tool, while the red cross marks indicate sequons that Re-Glyco deems unoccupied. b, The structural alignment of the CD16b (PDB 1E4J) in cyan with the CD16b (PDB 6EAQ, resolution 2.22 Å) in magenta. Both sequons occupied in PDB 6EAQ, namely, N45 and N162, are correctly predicted as occupied by Re-Glyco. c, The structure of the CD16b (PDB 1E4J) modified by swapping OD1 and ND2 coordinates and alternative rotameric orientation of the N74 sidechain N-glycosylated by Re-Glyco with a different selection of N-glycan structures from the GlycoShape GDB at each site. d, A close-up view of the OD1 and ND2 orientation of N45 in the CD16b from PDB 1E4J (cyan) and from PDB 6EAQ (magenta). e, A close-up of the orientation of the N74 sidechain in the CD16b from PDB 1E4J. The distance between the CG of N45 and the CA of F33 is 4.7 Å. Rendering of the 3D structures in a, b, d and e and rotamer search performed with pymol (https://pymol.org/2/). Rendering of 3D structure in c with VMD⁸⁶ (https://www.ks.uiuc.edu/Research/vmd/) and N-glycan 2D structures with DrawGlycan-SNFG⁸⁷ (http://www.virtualglycome.org/DrawGlycan/).