Extended Data Fig. 5: Least action path analysis of FUCCI-RPE1 cells profiled by scEU-seq. | Nature Methods

Extended Data Fig. 5: Least action path analysis of FUCCI-RPE1 cells profiled by scEU-seq.

From: Statistical inference with a manifold-constrained RNA velocity model uncovers cell cycle speed modulations

Extended Data Fig. 5

(a) RNA velocity streamline plots obtained with Dynamo8 using metabolically-labeled data (scEU-seq) for 2,793 RPE1 cells43, represented on the FUCCI-defined embedding space and colored by pseudotime (top) or FUCCI score (bottom). (b) Scatter-plot of cell cycle phase pseudotime from scvelo4 (top) and VeloCycle (bottom) compared to the ground truth FUCCI phase assignment acquired for RPE1 cells in (a). Pearson’s circular correlation coefficient is indicated in red. (c) Posterior estimate plot of velocity estimate for RPE1 cells obtained with VeloCycle. (d) Scatter-plot of per cell FUCCI signal (GMNN-GRP in green; CDT1-RFP in red) along the VeloCycle phase.

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