Extended Data Fig. 4: Histological analysis post chamber implant and laminectomy.
From: Long-term optical imaging of the spinal cord in awake behaving mice
a, Examples of EGFP+ fluorescence in naïve (a) and post-surgical (a', a'', and a''') CX3CR1-EGFP mouse spinal cord whole mounts and immunohistochemistry (coronal sections) with Neurotrace and anti-GFAP. Scale bars, 500 µm (whole-mount) and 200 µm (coronal slices). b, Spinal cord dissection and histology of a CX3CR1-EGFP mouse 1 week after chamber implantation. The whole-mount image (left) shows dorsal root ganglia in relation to the implant and the associated spinal segments. b’, cross-sectional views of EGFP (green) and GFAP staining (red) show minimal gliosis near the implant. Scale bars, 1 mm (whole-mount) and 200 µm (coronal slices). c, Quantification of microgliosis in naïve mice (n = 2) along with those after spinal chamber implant (1 week, n = 1) and laminectomy (1 week, n = 1 and 1 month, n = 2).
