Extended Data Fig. 7: Long-term imaging of cell bodies and axons in the spinal cord of awake mice.
From: Long-term optical imaging of the spinal cord in awake behaving mice
a, Clarity of GFP+ axons (Thy1-GFP mouse) with increasing sCMOS camera exposure times (LED power held constant). Yellow box: magnified section on the right. Yellow arrows: features with increased signal and minimal blur at 10-ms exposure. As a trade-off between SNR and clarity, we used 5–20-ms exposure times. Scale bars, 300 and 50 µm. b, Frames cropped to highlight cross-session matched areas from individual imaging sessions from a Thy1-GFP animal. Scale bar, 200 µm. c, Spearman correlation coefficient to the mean frame of a raw movie from a Thy1-GFP mouse (as in b). d, Increase in tdTomato expression in the dorsal columns after retro-orbital injection of AAV-PHP.S-tdTomato. Day 56, shows 10- and 100-ms exposure. Scale bar, 300 µm. e, Near daily imaging of GFP and tdTomato fluorescence normalized to baseline (pre retro-orbital injection). Magnified view of Fig. 3m highlights tdTomato signal increase from baseline.
