Extended Data Fig. 1: Awake spinal imaging experimental overview and designs.

a, Spinal cord imaging workflow. Several steps, such as microCT validation, are optional. b, Spinal implant chamber components: 3D printed and laser cut stainless or mild steel side bars, stabilizing plates, and the protective snap-on cover. Scale bar, 1 cm. c, Spinal cord surgery setup made from commercially available components and 3D printed parts, see Supplementary Table 4 for a parts list. d, Side bars technical diagram; units in mm. e, Stabilizing plate technical diagram; units in mm. f, Several (#1–6) iterative designs (top row, CAD; bottom row, real image) of the stabilizing plate with different positioning of the clamping/handling tabs. Side bars are included for size comparison. Scale bar, 1 cm. g, Horizontal view of the spinal cord implant chamber and optional screws (3D model). h, Spinal implant chamber (see f) with miniature screws. i, Protective cover for the spinal window (3D model); colors as in Fig. 1a. i”, magnified view of the cover (semi-transparent for visualization) on the spinal implant. j, Technical diagram of side bar cover; units in mm. k, Coronal view of an implant. Screws are optional. Note the dorsal-oriented attachment of the metal chamber components (red and blue pieces) to the T12-L1 vertebrae, compared to prior strategies (green pieces). Side clamps are used to manipulate the chamber during surgery and imaging. Colors for items are the same as in Fig. 1a. l, Survival curves (Kaplan-Meier estimator), as in Fig. 1g, illustrate the fibrosis onset probability PRECLUDE + Teflon AF (n = 36) or only Kwik-Sil (n = 10) surgeries; Kwik-Sil curve is not at zero (blue arrow) as n = 2 mice were fibrosis free or deceased at time of analysis. Censored data points indicate mice that died (X) or are still alive (circle) at the time of analysis. The purple arrow indicates time points with multiple alive mice.